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Engineered Biosynthesis of the Peptide Antibiotic Bacitracin in the Surrogate HostBacillus subtilis
Journal of Biological Chemistry
Nonribosomal peptides are processed on multifunctional enzymes called nonribosomal peptide synthetases (NRPSs), whose modular multidomain arrangement allowed the rational design of new peptide products. However, the lack of natural competence and efficient transformation methods for most of nonribosomal peptide producer strains prevented the in vivo manipulation of these biosynthetic gene clusters. In this study, we present methods for the construction of a genetically engineered Bacillusdoi:10.1074/jbc.m104456200 pmid:11448966 fatcat:di7dyldq3jgwzd3vmafdzlmmja