Electroretinography in Rats and Effect of Anesthesia for the Test

Takanori IKEDA, Masayasu HIDAKA, Reiko OHMORI, Shinji NARUSE, Hideo MIYAZAWA, Hiroshi KUNO
1995 Animal Eye Research  
This study was performed to determine the reproducibiiity of electroretinograms(ERGs)and the effects of anesthesia on the ERG. Rats were held in the dark for 2 to 3 hours before examination. They were restrained under light ether anesthesia, and topical Benoxyl(0.4%oxybuprocaine hydrochloride)was applied topically to the eyes. A contact lens attached to a recording electrode was placed on the cornea. Ground and reference electrodes were placed on the ear and forehead, respectively. The a and け
more » ... waves of ERG were elicited by a single Xenon flash of O.6 joule at 30 cm from the surface of the eye, and were recorded using a Neuropack Four Mini(MEB−5304, NEC)with a sweep time of 20msec/div., sensitivity of 200 pt V/div., and high and low cuts of l kHz and l Hz. The amplitudes and implicit times of Ia1 and bl, waves obtained froln 22 rats were 446±55 μVand I8.7±0.9 msec, and 971±116μVand 55.7±4.O msec, respectively. The coefficient of variation was 12%for amplitude and 5 to 7%for implicit time, suggesting that implicit time was a more consistent parameter than the amplitude. The coefficients of variation of averages of ttatt wave amplitudes and implicit times in 5 rats on 3 different days were less than 10%,suggesting acceptable reproducibility. The effects of pentobarbital 40 mg/kg i.p. on ERG were detemined in 5 rats. There were 6% and 20%decreases in amplitude, and 26%and l 4%increases in implicit time of tla and 「b waves, respectively, in pentobarbital−treated rats as compared with pretest values. In addition, rats were also tested without anesthesia, and nearly steady ERGs could be obtained under one or above one hour dark adaptation. This method provides relatively consistent amplitudes and implicit times of, a11 and I,b waves, and is able to detect the effects of pentobarbital on the electroretinal activity. These data demonstrate that the ERG method is essential to determine electrophysiologically adverse effects of test compounds on the retina.
doi:10.11254/jscvo.14.3-4_161 fatcat:a3xp62bfljc47dgbdxljqjtfvy