Cardiomyocyte behavior on biodegradable polyurethane/gold nanocomposite scaffolds under electrical stimulation

Yasaman Ganji, Qian Li, Elgar Susanne Quabius, Martina Böttner, Christine Selhuber-Unkel, Mehran Kasra
2016 Materials Science and Engineering C: Materials for Biological Applications  
25 Following a myocardial infarction (MI), cardiomyocytes are replaced by scar tissue, which 26 decreases ventricular contractile function. Tissue engineering is a promising approach to 27 regenerate such damaged cardiomyocyte tissue. Engineered cardiac patches can be 28 fabricated by seeding a high density of cardiac cells onto a synthetic or natural porous 29 polymer. In this study, nanocomposite scaffolds made of gold nanotubes/nanowires 30 incorporated into biodegradable castor oil-based
more » ... yurethane were employed to make 31 micro-porous scaffolds. H9C2 cardiomyocyte cells were cultured on the scaffolds for one 32 day, and electrical stimulation was applied to improve cell communication and interaction 33 in neighboring pores. Cells on scaffolds were examined by fluorescence microscopy and 34 scanning electron microscopy, revealing that the combination of scaffold design and 35 electrical stimulation significantly increased cell confluency of H9C2 cells on the 36 scaffolds. Furthermore, we showed that the gene expression levels of Nkx2.5, atrial 37 natriuretic peptide (ANF) and natriuretic peptide precursor B (NPPB), which are functional 38 genes of the myocardium, were up-regulated by the incorporation of gold 39 nanotubes/nanowires into the polyurethane scaffolds, in particular after electrical 40 stimulation. 41 42
doi:10.1016/j.msec.2015.09.074 pmid:26652343 fatcat:fbchupxfpvhkjm7mzetuh3h47e