Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut

Kaitheri Edathil Rachana, Sudalaimuthu Asari Naganeeswaran, Thayale Purayil Fayas, Regi Jacob Thomas, Muliyar Krishna Rajesh
2016 Acta Botanica Croatica  
Coconut palms are highly susceptible to diseases caused by different pathogens, and replanting with resistant varieties is the best way to manage them. Obtaining a collection of resistance gene analogues (RGAs) is an effective strategy to identify genomic regions linked to disease resistance. We have successfully used a comparative genomics approach to amplify putative RGAs from the coconut root (wilt) disease resistant cultivar Chowghat Green Dwarf (CGD) by using primers designed based on
more » ... rved motifs of the NBS-LRR domain of the date palm. The amplified sequences were cloned, sequenced and characterized. The coconut RGAs had high identity to monocot NBS-LRRs. A complete structural analysis and 3-D modeling of the NBS domain of coconut RGA was also undertaken. Real-time quantitative polymerase chain reaction analysis indicated that the isolated coconut NBS-LRR class RGAs was expressed more in root (wilt) disease resistant genotypes than in susceptible ones. This study would provide a base for future efforts to map disease resistant traits in coconut.
doi:10.1515/botcro-2016-0003 fatcat:cn55ffkzfvf7xeaizodhrnq4q4