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AbstractAll cells must compartmentalize their intracellular space in order to properly function1. Syncytial cells face an additional challenge to this fundamental problem, which is to coordinate gene expression programs among many nuclei. Using the skeletal muscle fiber as a model, we systematically investigated the functional heterogeneity among nuclei inside a syncytium. We performed single nucleus RNA sequencing (snRNAseq) of isolated myonuclei from uninjured and regenerating muscle, whichdoi:10.1101/2020.04.14.041665 fatcat:woqygjjah5amjopbfag4mn6bpy