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Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers asdoi:10.1038/s41598-018-24472-2 pmid:29773857 pmcid:PMC5958081 fatcat:meoomz7r7beqxiabkyohod7hyu