In this study, the osteogenic differentiation of murine pre-osteoblasts cultured on patterned silicon was investigated. Specifically, micropatterns were fabricated in <100> orientation silicon wafers to create linear gratings with a width of either 2 µm (2 µm depth, 10 µm pitch) or 20 µm (2 µm depth, 30 µm pitch). Two subclones (4, 24) of the MC3T3-E1 osteoblast-like cell line were seeded on the micropatterns and cultured in osteogenic induction medium for 28 days. Cells cultured on planar

more »

... on served as the controls. Apatite formation was examined using synchrotron X-ray diffraction. Deposition of [002] and [211]/[112] apatite-like material was clearly observed in subclone 4 (strongly mineralizing) osteoblasts cultured on both micro patterns. Keywords: Osteoblast; Micropatterns; Apatite; XRD 2. Daley WP, Peters SB, Larsen M (2008) Extracellular matrix dynamics in development and regenerative medicine. J Cell Sci 121: 255-264. 3. Bustillo JM, Howe RT, Muller RS (1998) Surface micromachining for micro electro mechanical systems. Proceedings of the IEEE 86: 1552-1574. 4. Fu G, Soboyejo WO (2009) Cell/surface interactions of human osteosarcoma (HOS) cells and micro-patterned poly dimelthylsiloxane (PDMS) surfaces. Materials Science and Engineering: C 29: 2011-2018. Citation: Kathryn D, Hadjiargyrou M, Yizhi M (2014) Apatitic Deposition in Osteoblasts Cultured on Micropatterned Silicon. Bioceram Dev Appl 4: 75.