Expression in Cereal Plants of Genes That InactivateFusariumMycotoxins

Arisa HIGA, Makoto KIMURA, Kouhei MIMORI, Tetsuko OCHIAI-FUKUDA, Takeshi TOKAI, Naoko TAKAHASHI-ANDO, Takumi NISHIUCHI, Tomoko IGAWA, Makoto FUJIMURA, Hiroshi HAMAMOTO, Ron USAMI, Isamu YAMAGUCHI
2003 Bioscience, biotechnology and biochemistry  
Trichothecene 3-O-acetyltransferase (encoded by Tri101) inactivates the virulence factor of the cereal pathogen Fusarium graminearum. Zearalenone hydrolase (encoded by zhd101) detoxiˆes the oestrogenic mycotoxin produced by the same pathogen. These genes were introduced into a model monocotyledon rice plant to evaluate their usefulness for decontamination of mycotoxins. The strong and constitutive rice Act1 promoter did not cause accumulation of TRI101 protein in transgenic rice plants. In
more » ... ast, the same promoter was suitable for transgenic production of ZHD101 protein; so far,ˆve promising T0 plants have been generated. Low transgenic expression of Tri101 was suggested to be increased by addition of an Q enhancer sequence upstream of the start codon.
doi:10.1271/bbb.67.914 pmid:12784641 fatcat:wtclxcesafgkhdu35mumuegtau