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Study of the activity of DNA polymerases β and λ using 5-formyluridine containing DNA substrates
Biopolymers and Cell
Branch of the Russian Academy of Sciences 8, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090 email@example.com Aims. To investigate the TLS-activity of human DNA polymerases b and l (pols b and l) using 5-formyluridine (5-foU) containing DNA duplexes which are imitating the intermediates during replication of the leading DNA strand, and to study the influence of replication factors hRPA and hPCNA on this activity. Methods. The EMSA and the methods of enzyme's kinetics weredoi:10.7124/bc.000051 fatcat:ojymkflsy5fthc4h3dswffey7y