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The motivation of this research was to determine the flow interactions on the pulsation and to express the influence on the flow character in the rigid and flexible tube. The character of Newtonian liquid was measured with the Particle Image Velocimetry method (PIV). Here, we used glass tube and Tygon tube for our comparison. We build the circuit equipped with membrane pump for generating pulsatile flow. The results were analysed over the pulse period sampled in 10 time steps. The fluid flowdoi:10.1051/epjconf/201921302031 fatcat:xpact4qmxbff3gmi7e26uvxuyq