Purification and Partial Characterization of an Acidic α-Glucan–Protein Complex from the Fruiting Body ofPleurotus sajor-cajuand Its Effect on Macrophage Activation

Saranya SATITMANWIWAT, Khanok RATANAKHANOKCHAI, Natta LAOHAKUNJIT, Patthra PASON, Chakrit TACHAAPAIKOON, Khin Lay KYU
2012 Bioscience, biotechnology and biochemistry  
The aim of this study was to purify an acidicglucan-protein complex from the fruiting bodies of Pleurotus sajor-caju by using the cell wall-degrading enzymes, xylanase and cellulase. The acidic glucanprotein complex was separated from a polysaccharide extract by using DEAE Toyopearl 650M anionexchange and Sepharose CL-6B chromatography. Its homogeneity was ensured by high-performance sizeexclusion chromatography and agarose gel electrophoresis. The acidic glucan-protein complex had a molecular
more » ... eight of approximately 182 kDa. Fourier transform infrared spectroscopy of the acidic glucan-protein complex revealed an -glycosidic bond and the typical characteristics of polysaccharides and proteins. The amino acid composition of the protein moiety was dominated by proline, glycine, glutamic acid and aspartic acid, indicating that the protein was highly flexible and had a negative charge. Atomic force microscopy proved that the acidic -glucan-protein complex existed in a spherical conformation. The acidic -glucan-protein complex stimulated the activation of macrophages, including the production of nitric oxide and tumor necrosis factor-.
doi:10.1271/bbb.120371 pmid:23047100 fatcat:6trvc4srtfgglosfdxzj73pktu