Vergleichende Untersuchung zur Gewinnung vom partikuliertem Knochen im Rahmen von enossalen Implantationen

Tarek Kweider
2012
The objective of this study is the comparative evaluation of particulated bone extraction in the framework of enossal implants. The quality rating (vitality, collagen I synthesis, ALP activity) of cells derived from the bone debris gathered by means of two methods is compared. During the preparation of the implant site incurred bone chips are obtained and evaluated. On the one hand, this is performed under high speed with original cutters cooled down by isotonic solution and with the help of
more » ... with the help of the bone collector (bone TRAP®, the company Astra). On the other hand, the material is obtained with a drill for single use at 300 rpm low speed without water cooling. To this end, the recovered bone meal is weighed, cultivated in a nutrient solution, assessed and treated in a cell culture. The vitality of the recovered bone, which is critical for clinical applications is measured, based on different parameters after four weeks of cell culture. Regarding vitality and differentiation it can be shown that the bone debris can be considered equivalent in both methods. During the cultivation of the bone it occurs that despite prophylactic actions (e.g. , antibiotics in the medium) were performed factors like bacteria, fungal spores and blood cells can have a negative influence on the results. The subject of a further investigation is the effect of chlorhexidine on the vitality of the bone chips. The evaluation of cells derived from the bone debris after chlorhexidine treatment allows us to presume that though the bacterial contamination is reduced, the vitality of the cells is negatively influenced.
doi:10.5283/epub.24697 fatcat:pb3lrkjqdjbqxmewid5q7kmtkq