Effect of different levels of crude glycerol on the morphology and some pathogenic bacteria of the small intestine in male broilers
The gut health of poultry is closely associated with feeds and feeding. The experiment was conducted to the effect of crude glycerol addition to diets of male broilers on the bacterial microflora and morphology of their small intestines (duodenum, jejunum and ileum). A total of 120 Ross 308 broiler chicks received diets containing 0% (GLY 0), 4% (GLY 4) or 8% (GLY 8) crude glycerol for 42 days. The presence ofColiformbacteria andEnterobacteriawas reduced in the duodenal tract of the broilers of
... of the broilers of GLY 4 (P<0.001); however, the presence ofStaphylococci/Micrococciin the GLY 8 was reduced relative to the other groups (P<0.001). The presence ofSalmonella spp.decreased in conjunction with the increasing quantities of glycerol (P<0.001). Analysis of the data regarding gut morphology (epithelial cell thickness, villi length and width, and crypt length and width) indicated that the glycerol levels fed to the different groups of broilers represented statistically different results in the small intestine. In general, whereas the diet with 4% glycerol statistically affected the investigated parameters of the gut, the diet with 8% glycerol statistically affected some segments of the broiler intestines.