MOESM3 of Silencing markers are retained on pericentric heterochromatin during murine primordial germ cell development

Aristea Magaraki, Godfried Heijden, Esther Sleddens-Linkels, Leonidas Magarakis, Wiggert Cappellen, Antoine Peters, Joost Gribnau, Willy Baarends, Maureen Eijpe
2017 Figshare  
Additional file 3. Immunofluorescent analysis of HP1γ in paraffin sections using regular and extended fixation protocols. A HP1γ (green) signal is enriched at DAPI (blue)-dense regions of E10.5 and E11.5 PGCs and somatic cells using the regular fixation protocol. Thereafter, at E13.5, HP1γ could not be detected in male germ cells, while it was still present in E13.5 female germ cell nuclei. B Upon application of the extended fixation protocol, enrichment of HP1γ signal was observed in
more » ... served in pericentric heterochromatin of E10.5 and E11.5 PGCs. Similar to A, HP1γ could not be detected at pericentric heterochromatin of male E13.5 germ cells, while it was still present in E13.5 female germ cells. Note that in both protocols (A, B) HP1γ could not reproducibly be detected in pericentric heterochromatin of E13.5 somatic cells. For each stage, two embryos were analysed per fixation protocol and at least 20 PGC nuclei were recorded. E10.5 and E11.5 PGCs were marked with OCT4 (red). E13.5 male and female germ cells were identified by the presence of TRA98 (red). Representative images are shown with germ cells highlighted by dashed yellow circles, and scale bars represent 5 μm.
doi:10.6084/m9.figshare.c.3715030_d3.v1 fatcat:4nu3lwfalbgzxbwtr33ujuw4lq