Crystal structure of mammalian purple acid phosphatase

Luke W Guddat, Alan S McAlpine, David Hume, Susan Hamilton, John de Jersey, Jennifer L Martin
<span title="">1999</span> <i title="Elsevier BV"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/y4oayrlrkfebxgzkm3rrzcps2m" style="color: black;">Structure</a> </i> &nbsp;
Mammalian purple acid phosphatases are highly conserved binuclear metal-containing enzymes produced by osteoclasts, the cells that resorb bone. The enzyme is a target for drug design because there is strong evidence that it is involved in bone resorption. Results: The 1.55 Å resolution structure of pig purple acid phosphatase has been solved by multiple isomorphous replacement. The enzyme comprises two sandwiched β sheets flanked by α-helical segments. The molecule shows internal symmetry, with
more &raquo; ... the metal ions bound at the interface between the two halves. Conclusions: Despite less than 15% sequence identity, the protein fold resembles that of the catalytic domain of plant purple acid phosphatase and some serine/threonine protein phosphatases. The active-site regions of the mammalian and plant purple acid phosphatases differ significantly, however. The internal symmetry suggests that the binuclear centre evolved as a result of the combination of mononuclear ancestors. The structure of the mammalian enzyme provides a basis for antiosteoporotic drug design. Addresses:
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/s0969-2126(99)80100-2">doi:10.1016/s0969-2126(99)80100-2</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/10425678">pmid:10425678</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/sjhkulzrhnd2dpbcl3adrzdvoe">fatcat:sjhkulzrhnd2dpbcl3adrzdvoe</a> </span>
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20190412205639/https://core.ac.uk/download/pdf/82704331.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/a5/e9/a5e9586711b23adff2fb27847b9b42fd1a03b40a.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1016/s0969-2126(99)80100-2"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> elsevier.com </button> </a>