Avoidance behaviour testing of Eisenia andrei in biodegradable plastic environment

Zuzana Tichá, Pavel Mach, Markéta Žáková, Monika Vítězová
2013 Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis  
TICHÁ ZUZANA, MACH PAVEL, ŽÁKOVÁ MARKÉTA, VÍTĚZOVÁ MONIKA: Avoidance behaviour testing of Eisenia andrei in biodegradable plastic environment. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, LXI, No. 5, pp. 1423-1429 Avoidance behaviour test with the earthworms (ISO 17512-1:2008) is a rapid screening test for the evaluation of soil and the infl uence of pollutants and chemicals on the behaviour of earthworms. The purpose of the testing is to determine the avoidance
more » ... rmine the avoidance behaviour of earthworm (in this case Eisenia andrei was used) which can be used as an organism for the composting and occur naturally in soil environment. The methodology was modifi ed according to the needs of the avoidance behaviour testing of earthworms in biodegradable plastic environment. It is a biodegradable thermoplastic material Mater-Bi, which is produced from corn starch. Californian earthworm (Eisenia andrei) was chosen as a test organism. The two-chamber test was used in testing. 10 earthworms were used, which were exposed to a number of concentrations of the test substance, which was mixed into the compost environment. It was recorded both a positive result, avoidance reaction, as well as a negative result, non-avoidance reaction and also there was a case, where individuals prefer both substrates equally. Organisms showed no escape reaction and were fairly evenly distributed in both halves of the test vessel, it can be assessed that organisms prefer both substrates equally. In testing, the mortality was zero, none of the individuals died, at the conclusion of the test there were not found any dead individuals. Avoidance higher than 80 % didn't occur; it cannot be said that the substrate is toxic or degraded.
doi:10.11118/actaun201361051423 fatcat:6hj4cmdejbe33bgbwca7jf2edm