ReScan, a Multiplex Diagnostic Pipeline, Pans Human Sera for SARS-CoV-2 Antigens

Colin R. Zamecnik, Jayant V. Rajan, Kevin A. Yamauchi, Sabrina A. Mann, Rita P. Loudermilk, Gavin M. Sowa, Kelsey C. Zorn, Bonny D. Alvarenga, Christian Gaebler, Marina Caskey, Mars Stone, Philip J. Norris (+10 others)
2020 Cell Reports Medicine  
Comprehensive understanding of the serological response to SARS-CoV-2 infection is important for both pathophysiologic insight and diagnostic development. Here, we generate a pan-human coronavirus programmable phage display assay to perform proteome-wide profiling of coronavirus antigens enriched by 98 COVID-19 patient sera. Next, we employ ReScan, a method to efficiently sequester phage expressing the most immunogenic peptides and print them onto paper-based microarrays using acoustic liquid
more » ... ndling, which isolates and identifies 9 candidate antigens, 8 of which are derived from the 2 proteins used for SARS-CoV-2 serologic assays: spike and nucleocapsid proteins. After deployment in a high-throughput assay amenable to clinical lab settings, these antigens show improved specificity over a whole protein panel. This proof-of-concept study demonstrates that ReScan will have broad applicability for other emerging infectious diseases or autoimmune diseases that lack a valid biomarker, enabling a seamless pipeline from antigen discovery to diagnostic using one recombinant protein source.
doi:10.1016/j.xcrm.2020.100123 pmid:32995758 pmcid:PMC7513813 fatcat:mej2qlomj5gilhh4qnez5oz6yu