The plant Tinospora cordifolia is well known in Ayurvedic medicine for its health benefits and clinical applications. Columbin, a natural diterpenoid is the major phytoconstituent isolated from the stem of Tinospora cordifolia. Various studies have investigated the anti-inflammatory, antimicrobial, antifeedant and cardio-protective roles of columbin. In this study, our centre of interest was to determine the anti-angiogenic activity of columbin using computational analyses and validating it

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... itro and in-vivo. Targeting dys-regulated angiogenesis can be a therapeutic intervention against various malignant, ischemic and inflammatory disorders. Molecular docking analyses showed favorable binding affinities of columbin to angiogenic receptors, VEGFR1 and VEGFR2. In-vitro experiments displayed columbin cytotoxicity on human umbilical vein endothelial cells (HUVECs) and also decreased endothelial migration and tubular formation. Invivo chick chorioallantoic membrane assay showed decreased vessel formation on columbin treatment. These findings altogether suggest that columbin can suppress angiogenesis in-vivo and in-vivo and may serve as a potential natural anti-angiogenic therapy.