Polymorphisms of the DNA Repair GenesXPDandXRCC1and the Risk of Age-Related Macular Degeneration

Ebru Görgün, Mehmet Güven, Mustafa Ünal, Bahadir Batar, Gülgün S. Güven, Melda Yenerel, Sinan Tatlipinar, Mehmet Seven, Adnan Yüksel
2010 Investigative Ophthalmology and Visual Science  
PURPOSE. Oxidative stress seems to be an important factor in the development of age-related macular degeneration (AMD). The role of DNA repair mechanisms has also received attention recently in AMD pathogenesis. This case-control study was conducted to determine the frequency of polymorphisms in two DNA repair enzyme genes, xeroderma pigmentosum complementation group D (XPD), codons 312 and 751, and x-ray cross-complementing group 1 (XRCC1), codons 194 and 399, in patients with AMD and in
more » ... th AMD and in disease-free control subjects. METHODS. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to analyze XPD Asp312Asn and Lys751Gln and XRCC1 Arg194Trp and Arg399Gln in 120 patients with AMD (65 with dry type and 55 with wet type) and in age-matched 205 disease-free control subjects. RESULTS. Genotypic and allelic distributions of the polymorphisms were detected. For the XPD polymorphism, although the allele frequencies were not different between the patients and healthy control subjects, there was a significant difference between frequencies for the XPD751 Gln/Gln genotype in AMD patients (9%) and healthy control subjects (19%; P ϭ 0.02). The XPD751 Gln/Gln genotype seemed to have a protective effect against development of AMD (odds ratio, 0.41; 95% confidence interval, 0.19 -0.88). Stratification by subtype of AMD revealed that the XPD751 Gln/Gln genotype was significantly lower only in the patients with dry type (P ϭ 0.02). These interactions remained nearly significant after Bonferroni correction (P Ͻ 0.0125). Haplotype analysis for the two XPD polymorphisms revealed that the haplotype GC ( 312 Asp-751 Gln) was a protective haplotype against AMD. No statistically significant difference was found for the genotypic and allelic distributions of the polymorphisms in the XRCC1 gene between the patients and the control subjects. CONCLUSIONS. Polymorphism in XPD codon 751 may be associated with the development of AMD. (Invest Ophthalmol Vis Sci. 2010;51:4732-4737)
doi:10.1167/iovs.09-4842 pmid:20375340 fatcat:525y3gtcqfh5hpot2mooqjysfa