Antiepileptic Potential of Ursolic Acid Stearoyl Glucoside by GABA Receptor Stimulation

Imran Kazmi, Muhammad Afzal, Gaurav Gupta, Firoz Anwar
2012 CNS Neuroscience & Therapeutics  
The antiepileptic potential of ursolic acids stearoyl glucoside (UASG) isolated from Lantana camara L. was evaluated against maximal electroshock (MES) induced and isoniazid (INH) induced seizures in experimental animal models. Ursolic acid is already known for CNS depressing, anticonvulsant and analgesic activity [1] . Dried powder of Lantana camara leaves (4 kg) was extracted with methanol (12 L) at 50°C for 24 h. The slurry obtained was dissolved in methanol, and further, it was adsorbed on
more » ... it was adsorbed on silica gel (60-120 mesh). The slurry was subjected to a silica gel column using CHCl3/MeOH gradient system (49:1; 2.0 L for gradient system); it yielded colorless crystals of USAG (yield 11.2 g, 0.28%) [2] . Wistar albino rats (150-200 g) were divided into four groups (n = 6). Group I (solvent control), received 0.9% (w/v) of saline (1 mL/100 g), Group II (positive control), received Diazepam (5 mg/kg) Group III and IV received UASG, 25 and 50 mg/kg suspended in 0.9% (w/v) of saline. Antiepileptic potential of UASG was established through INH and MES protocol-induced seizure. Isoniazid-induced seizure model, all animals were treated intraperitonially 30 min prior to administration of INH (300 mg/kg). Animals that did not convulse within 30 min were considered as protected and their results were expressed in terms of percentage. Group III and IV animals were monitored for 60 min for determination of percentage protection. In unprotected animals, the latency to first convulsion and the durations of convulsions were recorded [3] . Forty-five minute after vehicle or compound 1 and 30 min after diazepam, rats were sacrificed. The animals of group III were sacrificed as soon as onset of convulsions occurs or 65 second after INH treatment. Brain tissue was isolated and transferred Table 1 Effect of Ursolic acid stearoyl glucoside on isoniazid (INH)-induced seizure in rats Treatment (dose, mg/kg, i.p.) No. convulsed/ no. used Animals not convulsed (% animals protected) Seizure latency (s) Seizure duration (s) Mortality (%) GABA level (ng/g of brain tissue)
doi:10.1111/j.1755-5949.2012.00369.x pmid:22943148 fatcat:inm3ngteofg6rd236d6ztshjme