Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model
The Medical journal of Cairo University
Renal nerves and Renin-Angiotensin Aldosterone System (RAAS) are involved in the early renal pathological changes occurring during the development of Diabetic Nephropathy (DN). Bilateral renal nerve denervation (BRD) and angiotensin II (AngII) blockade have renoprotective effects by retarding the progression of renal fibrosis through Transforming growth factor β 1 (TGF-β 1) signaling. This study aimed to compare therole of AngII and renal nerves on TGF-β/Smadpathway in diabetic nephropathy rat
... ic nephropathy rat model. Material and Methods: Eightymale Sprague-Dawley rats divided into 4 groupswere used: Group 1 (normal control group), group II (untreated diabetic nephropathy group), group III (diabetic nephropathytreated by valsartan), group IV (diabetic nephropathytreated by renal denervation). Rats were assessed by measuring Blood Pressure (BP), blood glucose, body and kidney weights, fluid and food intake and urine volume/24h, renal function tests, mRNA expression and activity of TGF-β 1 and Smad3 and histopathological changes in all groups at the end of fourth week of valsartan administration and BRD operation. Results: Valsartan and BRD treatment significantlyimproved renal functionand reduced ABP, blood glucose level, histopathological score and TGF-β 1 and Smad3 and their mRNA expression with significant difference between them and the control and untreated groups. Conclusion: Valsartan appeared to alleviate DN by suppressing TGF-β 1 and ph-Smad3. This also occurs in case of renal denervation but to a lesser degree.