Zinc Determines Dynamical Properties and Aggregation Kinetics of Human Insulin [article]

Kevin Pounot, Geoffrey W. Grime, Alessandro Longo, Michaela Zamponi, Daria Noferini, Viviana Cristiglio, Tilo Seydel, Elspeth Frances Garman, Martin Weik, Vito Fodera, Giorgio Schiro
2020 bioRxiv   pre-print
Protein aggregation is a widespread process leading to deleterious consequences in the organism, with amyloid aggregates being important not only in biology but also for drug design and biomaterial production. Insulin is a protein largely used in diabetes treatment and its amyloid aggregation is at the basis of the so-called insulin-derived amyloidosis. Here we uncover the major role of zinc in both insulin dynamics and aggregation kinetics at low pH, where the formation of different amyloid
more » ... erstructures (fibrils and spherulites) can be thermally induced. Amyloid aggregation is accompanied by zinc release and the suppression of water-sustained insulin dynamics, as shown by particle-induced X-ray emission and X-ray absorption spectroscopy and by neutron spectroscopy, respectively. Our study shows that zinc binding stabilizes the native form of insulin by facilitating hydration of this hydrophobic protein and suggests that introducing new binding sites for zinc can improve insulin stability and tune its aggregation propensity.
doi:10.1101/2020.07.02.184903 fatcat:f6jg23l55bg4rl3zadxlpul2im