Observation of subcellular distribution of antisense oligonucleotidel lipid complexes by confocal laser scanning microscope Distribution of phosphorothioate antisense oligonucleotide (S-oligo), S-oligo/Iipofectin com plex and S-^ligo/PEG lipid complex in Hela cells was observed by a confocal laser scanning micro scope. The S-oligo localized to endosome/lysosome in a punctate fluorescence pattern. In the presence of lipofectin, the S-oligo was accumulated in the nucleus of Hela cells. The

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... /PEG lipid com plex showed both punctate fluorescence and diffuse fluorescence in the cyoplasm, but not in the nucleus. These result clearly demonstrated that the sub cellular distribution of S-oligo depended on the delivery system.