Evolutionarily conserved RNA structures in the upstream regions of Wuhan seafood market pneumonia virus (Wuhan-nCoV) and SARS virus [graphic]

Michael Wolfinger
2020 Figshare  
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 11.0px Monaco; color: #000000; background-color: #e1e2be} span.s1 {font-variant-ligatures: no-common-ligatures} p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 11.0px Monaco; color: #000000; background-color: #e1e2be} span.s1 {font-variant-ligatures: no-common-ligatures} In silico prediction of evolutionarily conserved RNA secondary structures in the upstream 500 nucleotides of Wuhan seafood market pneumonia virus (Wuhan-nCoV, GenBank MN908947.3, top)
more » ... MN908947.3, top) and severe acute respiratory syndrome virus (SARS, RefSeq NC_004718.3, bottom). The genomic regions shown here comprise the 5'UTR and the amino-terminal region of the nonstructural protein 1 (highlighted in orange). A small upstream ORF (uORF) is found in both 5'UTRs and is highlighted in blue.Phylogenetic proximity of Wuhan-nCoV and SARS is reflected by several homologous stem-loop structures, as inferred from covariance model analysis. In particular, stem-loops SL1, SL2, SL3, SL4, SL5, SL5a, SL5b, SL5c, SL6 and SL7 show a high degree of structural similarity. Wuhan-nCov is predicted to from an additional small stem loop structure SL4a, as well as a slightly extended SL8.Structures were predicted plotted with the ViennaRNA Package v2.4.14 (https://www.tbi.univie.ac.at/RNA/)
doi:10.6084/m9.figshare.11659575.v1 fatcat:yopflnr3ozeihj3hip6o52buke