Fast volumetric mapping of human brain slices

Luca Pesce, Annunziatina Laurino, Vladislav Gavryusev, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, Francesco Saverio Pavone, Qingming Luo, Jun Ding (+1 others)
2020 Neural Imaging and Sensing 2020  
and Vladislav Gavryusev contributed equally to this work Keywords: Light sheet microscopy, whole brain imaging, quantitative imaging, tissue clearing, human brain Abstract. We still lack a detailed map of the anatomical disposition of neurons in the human brain. A complete map would be an important step for deeply understanding the brain function, providing anatomical information useful to decipher the neuronal pattern in healthy and diseased conditions. Here, we present several important
more » ... es towards this goal, obtained by combining a new clearing method, advanced Light Sheet Microscopy and automated machine-learning based image analysis. We perform volumetric imaging of large sequentially stained human brain slices, labelled for two different neuronal markers NeuN and GAD67, discriminating the inhibitory population and reconstructing the brain connectivity. Neurophotonics, edited by
doi:10.1117/12.2544133 fatcat:2u3r4aggbvbahhs5ornl6eouuu