Characterization of macrolide resistance in Gram-positive cocci from Colombian hospitals: a countrywide surveillance

Jinnethe Reyes, Marylin Hidalgo, Lorena Díaz, Sandra Rincón, Jaime Moreno, Natasha Vanegas, Elizabeth Castañeda, César A. Arias
2007 International Journal of Infectious Diseases  
Objective: The characterization of macrolide resistance in Gram-positive cocci recovered from Colombian hospitals. Methods: The resistance profiles and mechanism of macrolide resistance were investigated in isolates of Streptococcus pneumoniae (1679), Staphylococcus aureus (348), coagulase-negative staphylococci (CoNS) (175), and Enterococcus spp (123). Minimum inhibitory concentrations (MICs) for erythromycin (ERY) and clindamycin (CLI), detection of macrolide resistance genes, phenotypic
more » ... es, phenotypic characterization, and pulsed field gel electrophoresis (PFGE) of macrolide-resistant pneumococci were performed. Results: Resistance to ERY and CLI was 3.3% and 2.3% for S. pneumoniae, 58% and 57% for S. aureus (94% for both compounds in methicillin-resistant Staphylococcus aureus (MRSA)), and 78.6% and 60.7% in methicillin-resistant Staphylococcus epidermidis, respectively. ERY resistance was 62% in Enterococcus faecalis and 82% in Enterococcus faecium. The MLS B -type accounted for 71% of S. pneumoniae and 100% of MRSA. The erm(A) gene was prevalent in MRSA, erm(B) in S. pneumoniae and enterococci, and erm(C) in CoNS isolates. Efflux pump genes (mef(A) genes) were mostly identified in S. pneumoniae (24%). The most common genotype amongst ERYresistant pneumococci was the Spain 6B -2 clone. Conclusions: The prevalence of macrolide resistance is low in Colombian pneumococci and high in MRSA (cMLS B -type). #
doi:10.1016/j.ijid.2006.09.005 pmid:17320446 fatcat:n7cf3lvcanfezh5acbsp53uvyy