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Construction of stable cloning vectors that do not segregate from a human fecal Escherichia coli strain in the streptomycin-treated mouse large intestine
Infection and Immunity
Escherichia coli F-18 Colwas previously shown to be a poor colonizer of the streptomycin-treated mouse large intestine, relative to its parent, E. coli F-18. Prior to attempting to clone genes responsible for the colonization phenotype of E. coli F-18 into E. coli F-18 Col-, a suitable cloning vector had to be found. In this investigation, we report that the commonly used cloning vectors pBR322, pHC79, and pBR329 all segregate from E. coli F-18 Colboth when grown in L broth under conditions ofdoi:10.1128/iai.58.5.1141-1145.1990 fatcat:63mwlaadefc25d6ujtvoidxirq