Runx3-mediated Transcriptional Program in Cytotoxic Lymphocytes

Joseph Lotem, Ditsa Levanon, Varda Negreanu, Dena Leshkowitz, Gilgi Friedlander, Yoram Groner, Jose Alberola-Ila
<span title="2013-11-13">2013</span> <i title="Public Library of Science (PLoS)"> <a target="_blank" rel="noopener" href="https://fatcat.wiki/container/s3gm7274mfe6fcs7e3jterqlri" style="color: black;">PLoS ONE</a> </i> &nbsp;
The transcription factor Runx3 is highly expressed in CD8 + T and NK cytotoxic lymphocytes and is required for their effective activation and proliferation but molecular insights into the transcription program regulated by Runx3 in these cells are still missing. Using Runx3-ChIP-seq and transcriptome analysis of wild type vs. Runx3 -/-primary cells we have now identified Runx3-regulated genes in the two cell types at both resting and IL-2-activated states. Runx3bound genomic regions in both
more &raquo; ... types were distantly located relative to gene transcription start sites and were enriched for RUNX and ETS motifs. Bound genomic regions significantly overlapped T-bet and p300-bound enhancer regions in Runx3-expressing Th1 helper cells. Compared to resting cells, IL-2-activated CD8 + T and NK cells contain three times more Runx3-regulated genes that are common to both cell types. Functional annotation of shared CD8 + T and NK Runx3-regulated genes revealed enrichment for immune-associated terms including lymphocyte activation, proliferation, cytotoxicity, migration and cytokine production, highlighting the role of Runx3 in CD8 + T and NK activated cells. Citation: Lotem J, Levanon D, Negreanu V, Leshkowitz D, Friedlander G, et al. (2013) Runx3-mediated Transcriptional Program in Cytotoxic Lymphocytes. PLoS ONE 8(11): e80467.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1371/journal.pone.0080467">doi:10.1371/journal.pone.0080467</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/24236182">pmid:24236182</a> <a target="_blank" rel="external noopener" href="https://pubmed.ncbi.nlm.nih.gov/PMC3827420/">pmcid:PMC3827420</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/lurxrzjog5bxblt7zr5xqz72wa">fatcat:lurxrzjog5bxblt7zr5xqz72wa</a> </span>
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