Galangal essential oil (EO) has a promising impact as a rumen modifier because it contains 24% of cineole and known to have antimicrobial activity on Gram positive and Gram negative bacteria. Improving the quality of milk composition can be achieved by modification of microbial abundance in the rumen. The study was conducted to measure milk production and composition of Holstein Friesian cow ration, which was supplemented with galangal (EO). The experiment was designed a completely randomized

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... sign, 60% of mott elephant grass and 40% of concentrate (dry matter basis) were supplemented by galangal EO as much as 0, 1.25 and 2.50 g/head/day with four replications. The data on milk production were collected every day of milking. Milk composition (lactose, protein, and fat) were analyzed each week for 30 days, and milk fatty acids were analyzed at the end of the research. The results showed no significant difference in milk production, milk composition among rations. Furthermore, milk urea nitrogen (MUN) and blood urea nitrogen (BUN) concentration was also absorbed and galangal EO did not effect on both treatments. Galangal EO at dose of 1.25 g/head/day decreased significantly on blood of volatile fatty acids. The concentration of unsaturated fatty acids (ɣ-linolenic acids) ratio 1.25 was higher than 0 and 250 g/head/day. In conclusion, Galangal EO supplementation increased the polyunsaturated fatty acids ratio at level 1.25 g/head/day of galangal EO, but did not affect milk production, lactose, protein, and fat content.