Internet Archive Scholar

Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells

Shoma Tamori, Yuka Nozaki, Hitomi Motomura, Hiromi Nakane, Reika Katayama, Chotaro Onaga, Eriko Kikuchi, Nami Shimada, Yuhei Suzuki, Mei Noike, Yasushi Hara, Keiko Sato (+9 others)
2018 OncoTarget  

Preserved Fulltext

fulltext thumbnail
Web Archive Capture PDF (3.1 MB)
https://web.archive.org/web/20190430031231/http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=download&path%5B%5D=26369&path%5B%5D=82122

A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2019; you can also visit the original URL. The file type is application/pdf.

Glyoxalase 1 (GLO1) is a ubiquitous enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis that induces apoptosis. In this study, we found that GLO1 gene expression correlates with neoplasm histologic grade (χ 2 test, p = 0.002) and is elevated in human basal-like breast cancer tissues. Approximately 90% of basal-like cancers were grade 3 tumors highly expressing both GLO1 and the cancer stem cell marker ALDH1A3. ALDH1high cells derived from the MDA-MB 157
more » ... d MDA-MB 468 human basal-like breast cancer cell lines showed elevated GLO1 activity. GLO1 inhibition using TLSC702 suppressed ALDH1high cell viability as well as the formation of tumor-spheres by ALDH1high cells. GLO1 knockdown using specific siRNAs also suppressed ALDH1high cell viability, and both TLSC702 and GLO1 siRNA induced apoptosis in ALDH1high cells. These results suggest GLO1 is essential for the survival of ALDH1-positive breast cancer stem cells. We therefore conclude that GLO1 is a potential therapeutic target for treatment of basal-like breast cancers.
doi:10.18632/oncotarget.26369 pmid:30559934 pmcid:PMC6284866 fatcat:st7xpheh5zhufmfd4r4tijc5cq
Open Access
Citation

Shoma Tamori, Yuka Nozaki, Hitomi Motomura, Hiromi Nakane, Reika Katayama, Chotaro Onaga, Eriko Kikuchi, Nami Shimada, Yuhei Suzuki, Mei Noike, Yasushi Hara, Keiko Sato, Tsugumichi Sato, Kouji Yamamoto, Takehisa Hanawa, Misa Imai, Ryo Abe, Atsushi Yoshimori, Ryoko Takasawa, Sei-Ichi Tanuma, Kazunori Akimoto. "Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells." OncoTarget 9.92 (2018) 36515-36529