220ml of blood from each healthy donor was collectedinto a triple-plastic bag containing ACD or CPD anticoagulants. Platelet concentrates (PC) with approximate volumes of 20ml were prepared by centrifugation. Each PC was divided into two portions and then stored at 22C with or without agitation. pHs of plasma, cell volumes, aggregabilities, shape change abilities and shapes of platelets on PC were studied after storage of 0-72hours. The initial pH of ACD-PC was 7.2 and that of CPD-PC was 7.45.

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... uring 72 hours storage, pHs of plasma in both anticoagulants-PC without agitation, decreased to 7.0. However, pH values of plasma in agitated PC increased to 7.5-7.6.pH values of CPD-PC were always higher than ACD ones. On the other hand, differences on morphological changes of platelet, the average values of platelet MPV (mean particle volume), aggregabilities by ADP or collagen and shape change abilities were not observed in two anticoagulants-PC during the periods of experiments. Storage of platelets by agitation usually resulted in better preservation of platelets with regard to discoid shape, MPV, shape change abilities than observed with nonagitated storage. The data suggest that gentle agitation (30 cycles/min. on a platform rotator) should be necessary for better storage of PC using ACD or CPD anticoagulants.