Plantlet Regeneration through Indirect Organogenesis of Flame Gold Tree (Koelreuteria elegans Laxm.)

Rameshwar Groach, Muzafar Hussain Dar, Kartar Chand Badgal, Priyanka Pal, Narender Singh, Kuldeep Yadav
2014 Journal of Ornamental Plants   unpublished
Koelreuteria elegans, popularly known as "Flame Gold" is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration (70%) and development (4.35 shoots). MS half strength medium supplemented with 0.2 mg/l NAA reported
more » ... 0% rooting after 21 days of implantation. Mostly, the roots were long and healthy. Plants were successfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1) with 65% survival rate under field conditions. The in vitro regenerated plantlets were hardened and acclimatized successfully.