MicroRNA and messenger RNA As Potential Urinary Biomarkers in Prostate Cancer

2020 Journal of Urology & Nephrology  
Prostate Cancer (PCa) is the fifth leading cause of death world wide and these condmost common cancerinmen. Studies to search for new biomarkers,especially with non-invasivemethods, are carried out, one of which is urinary biomarker as an early detection and predictors of PC aprognosis. microRNA (miRNA) and messenger RNA(mRNA) has proven to have important roles in various oncogenic processes. Material and methods: Urine samples collected from 145 patients were examined,45 patients diagnosed
more » ... Benign Prostate Hyperplasia (BPH) and 100 patients diagnosed with PCa.Urine samples were collected from each patient and examined in the biomolecular laboratory.The geneexpression were analyze dusing qPCR analysis using the qPCRCFX 96 thermocycler (Bio-Rad). Results: The expression of miR-21-5p was higher in BPH group compared to PC a groups, both non-metastatic and metastatic, with p-values of 0.004 and 0.017, respectively. BPH showed the highest mRNA expression of PDCD-4. Conclusion: The overexpression of miR-21-5p shown in this study could be a potential non-invasive diagnostic tool for patients with PCa. The lower expression mRNA of PDCD-4 in non-metastatic compared to metastatic PCa group could be potential prognostic biomarker in PCa.
doi:10.13188/2380-0585.1000029 fatcat:ymuhdrduifblzajiajvbrqope4