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Engei Gakkai zasshi
In apple fruit, three types of sorbitol enzymes were certified by column chromatography using Sephadex G-200 gel. The first type was sorbitol-6-phosphate dehydrogenase catalyzing the conversion between sorbitol-6-phosphate and glucose-6-phosphate, which was active in the alkaline region (optimum pH 9.8) and a Km value of 7 mM for sorbitol-6-phosphate. The second type was sorbitol dehydrogenase interconverting sorbitol and fructose, having optimum activity at pH 9.8 and a Km value of 125 mM fordoi:10.2503/jjshs.49.429 fatcat:whtkzfpjurhlfp7xrmzyf6emwi