Reproduction and endoreduplication in Drosophila melanogaster Meig. when influenced by lead nitrate
Faktori eksperimentalʹnoï evolûcìï organìzmìv
Aim. The purpose of investigation was to study the reproductive ability and polyteny degree of chromosomes in Drosophila melanogaster Meig. under the influence of various concentrations of lead nitrate. Methods. Canton-S wild-type strain was used as the material. Flies developed on standard sugar-yeast medium, to which in the experiment lead nitrate was added in concentrations of 0.1, 1, and 10 mg/ml. The reproductive ability of the strain was evaluated by the number of adult offspring. The
... offspring. The polyteny degree of chromosomes was studied on squashed preparations of larva salivary glands stained with acetoorsein by cytomorphometry. The preparations were obtained at late 3rd instar. Results. The number of adult offsprings decreased when lead nitrate was added to the nutrient medium: at a concentration of 0.1 mg/ml – by 22.8 %, at 1 mg/ml – by 38.9 %. A concentration of 10 mg/ml was lethal. Males showed greater sensitivity to the drug compared to females. The degree of polyteny of chromosomes in the salivary glands of larvae decreased on average by 5.0–6.5 %. Conclusions. Lead nitrate causes a significant, dose-dependent decrease in the reproductive ability of fruit flies and has a toxic effect on Drosophila cells, inhibiting the process of endoreduplication. Keywords: Drosophila melanogaster Meig., heavy metals, fecundity, giant chromosomes, polyteny.