Transcription of the major Drosophila heat-shock gene in vitro

Brian L. Craine, Thomas Kornberg
1981 Biochemistry  
Active eukaryotic genes are more accessible to some proteins that bind DNA than are inactive genes. In order to probe the accessibility of the Drosophila heat-shock genes, we have isolated nuclei from Drosophila tissue culture cells and have used these nuclei as templates for Escherichia coli RNA polymerase. With nuclei isolated from cells that had not been heat shocked, the synthesis of heat-shock RNA was not detected by hybridization to a DNA clone containing sequences from the major
more » ... the major heat-shock region. In contrast, approximately 0.22% of the RNA synthesized in nuclei isolated E v i d e n c e from numerous sources has documented the structural differences between actively transcribed and inactive chromatin. In some cases, transcriptionally active or inactive chromatin can be distinguished by gross morphology. Three
doi:10.1021/bi00526a010 pmid:6272841 fatcat:iubhatsi5rh7dljrgy2qjnykhe