Evaluation ofMycobacterium lepraeparticle agglutination test, using eluates of filter paper blood spots
A comparison of the ELISA test with the newly-developed MLPA test was carried out, using eluates of blood spots from filter paper for the detection of the anti-PGL-I antibody. A very good positive correlation was observed between these two tests. The concordance rate was fo und to be 92,6%, ranging from 71-4% to 100%. This nonconcordance was not fo und when freshly-collected samples were used. The MLPA test is simple and reliable. The use of eluates from blood spots collected on filter paper fu
... on filter paper fu rther simplifies the test in the collection and transportation of blood samples. This accurate and rapid method makes the MLPA test logistically fe asible for large-scale screening. With our modification of MLPA with eluates more samples can be screened with the kit than with sera. Since the introduction of ELISA using phenolic glycolipid-II• 2 (PGL-I) serodiagnosis of leprosy has undergone changes over many years. Because many synthetic glycoconjugates of PGL-I with terminal disaccharide-3-6 or trisaccharide-based antigens are available,4.7,8 ELISA fo r anti PGL-I has been refined for serodiagnosis of leprosy. A Latex agglutination test has also been developed for rapid serodiagnosis of leprosy.9 There has recently been developed a novel gelatin agglutination test named the My cobacterium /eprae particle agglutination (MLPA) test that uses sensitized gelatin particles.1o This test was fo und to be simple, and had a sensitivity and specificity comparable to those of conventional anti-PGL-I ELISA, and was further fo und to be useful in the field in leprosy-endemic countries. We present here our experiences with this simple test and our attempt to fu rther simplify the test by using eluates of blood spots, collected on filter paper.