Crystal structure determination of Cys-S-sulfonated HsGAPDH from protein purified from the supernatant of HEK293F cells [report]

Pietro Roversi, Andrea Lia
2020 Zenodo  
An account of the experimental steps taken to obtain crystal structures of human GAPDH which show partial oxidation of the catalytic cysteine of the protein to cysteine S-sulfonic acid. The context to the experiments is briefly described at our Open Secretion Rescue blog hosted at the SGC Open Lab Notebooks.
doi:10.5281/zenodo.3778536 fatcat:cjy5w5l3r5cptf343felohvk4u