Histopathological evaluation of potential impact of β-tricalcium phosphate (HA+ β-TCP) granules on healing of segmental femur bone defect
H. Eftekhari, M. R. Farahpour, S. M. Rabiee
Bratislava Medical Journal
Histopathological evaluation of β-tricalcium phosphate (HA+ β-TCP) granules demonstrated that it has properties to heal segmental femur bone defect in rat. In this study, 27 male white rats were examined. Rats were divided into tree groups. Surgical procedures were done after IP administration of ketamine 5 % and xylazine HCL 2 %. Then an approximately 5-mm long, 3-mm deep and 2-mm wide bone defect was created in the femur of one of the hind limbs using a No. 0.14 round bur. After inducing the
... urgical wound, all rats were colored and randomly divided into three experimental groups of nine animals each: Group 1 received medical pure β-tricalcium phosphate granules, group 2 received hydroxyapatite and third group was a control group with no treatment. Histopathological evaluation was performed on days 15, 30 and 45 after surgery. On day 45 after surgery, the quantity of newly formed lamellar bone in the healing site in β-TCP group was better than onward compared to HA and control groups. In conclusion, β-tri calcium phosphate (β-TCP) granules exhibited a reproducible bone-healing potential (Fig. 10, Ref. 28) . Text in PDF www.elis.sk. Acknowledgements: The authors are grateful to Doostar (Ph.D.) for histopathology assessment. Kawasaki T, Takahashi S, Ideda K. Hydroxyapatite high-performance liquid chromatography: column performance for proteins. European Journal of Biochemistry. 1985; 152 (2): 361-71. 24. Schenk R, Buser D, Hardwick WR, Dahlin C. Healing pattern of bone regeneration in membrane-protected defects: a histologic study in the canine mandible. Intern J Oral Maxillofac Implants 1994; 9 (1): 13. 25. Story BJ, Burgess AV, La D, Wagner WR. In vitro st ability of a highly crystalline hydroxylapatite coating in a saturated citric acid solution. J Biomed Mat Res 1999; 48 (6): 841-847. 26. Daculsi G, LeGeros R. In Handbook of Bioceramics an d their Applications; T. Kokubo. Woodhead Publishing Ltd., London; 2008. 27. LeGeros RZ. Calcium phosphates in oral biology and medicine. Monographs Oral Sci 1990; 15: 1-201. 28. Chang YL, Stanford CM, Keller JC. Calcium and phosph ate supplementation promotes bone cell mineralization: Implications for hydroxyapatite (HA)-enhanced bone formation.