e0311 Prevalence of cardiovascular disease biological risk factor clustering among overweight and obese population in Beijing community--results from CCEIP

F. Yuanyuan, Y. Jinming, W. Jiahong, S. Yihong, L. Huifang, H. Dayi
2010 Heart  
cellular response to insulin, VSMCs from WKY and SHR were isolated and cultured, and its proteome were comparatively analysed with normal control by 2-DE. Results showed that the proliferation of VSMCs from SHR be more sensitive to insulin stimulation than that VSMCs from WKY. The detectable spots ranged from 537 to 608 on the gels in VSMCs of SHR, and 413631 spots in VSMCs of WKY. The different expressed protein spots in VSMCs of SHR were then isolated and measured by MALDI-TOF-MS. A total of
more » ... 8 spots showed a sharp clear spectrum, and 13 spots matched with the known proteins from database. These proteins were mainly involved in cytoskeleton, glycometabolism and post-translational processes. Among these proteins, OPN and matrix gla protein were up-regulated expression proteins, whiled a-SM actin was down-
doi:10.1136/hrt.2010.208967.311 fatcat:rwxlrwhx3jhpzlh2fq757qwinu