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C5a Receptor Activation
Journal of Biological Chemistry
Hormones and sensory stimuli activate serpentine receptors, transmembrane switches that relay signals to heterotrimeric guanine nucleotide-binding proteins (G proteins). To understand the switch mechanism, we subjected 93 amino acids in transmembrane helices III, V, VI, and VII of the human chemoattractant C5a receptor to random saturation mutagenesis. A yeast selection identified 121 functioning mutant receptors, containing a total of 523 amino acid substitutions. Conserved hydrophobicdoi:10.1074/jbc.274.22.15757 pmid:10336477 fatcat:4u6bczn64neurcip3ifi4hyciq