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The process of turning 2D micrographs into 3D atomic models of the imaged macromolecules has been under rapid development and scrutiny in the field of cryo-EM. Here, some important methods for validation at several stages in this process are described. Firstly, how Fourier shell correlation of two independent maps and phase randomization beyond a certain frequency address the assessment of map resolution is reviewed. Techniques for local resolution estimation and map sharpening are also toucheddoi:10.1107/s2059798321006069 pmid:34473085 pmcid:PMC8411978 fatcat:ikwmefph2rdexo62iuqmqi2ppm