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Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering
2009
Analytical Sciences
Preserved Fulltext
Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (DIRLS) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 mg mL -1 , and
doi:10.2116/analsci.25.727
pmid:19430161
fatcat:fkt4xyfqybfhhaq2wkt6ktzrpm