Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

Juan DU, Huzhi ZHENG, Chengzhi HUANG, Congying WEN, Jianyu HAO, Yufei HU, Dongbo ZHOU, Li LIU
2009 Analytical Sciences  
Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (DIRLS) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 mg mL -1 , and
more » ... the detection limit (S/N = 3) was 17.0 ng mL -1 . The binding ratio and the binding constant between DNA and polymer were also provided.
doi:10.2116/analsci.25.727 pmid:19430161 fatcat:fkt4xyfqybfhhaq2wkt6ktzrpm