DETERMINATION OF NAPROXEN SODIUM FROM POLY(LACTIDE-CO-GLYCOLIDE) CORNEAL SCAFFOLDS

Seren KAYIRAN, Sibel BOZDAĞ PEHLİVAN, Mustafa ÇELEBİER, Nursen ÜNLÜ
2010 Turkish Journal of Pharmaceutical Sciences  
A simple, very specific, and reliable HPLC method has been developed and validated for the determination of naproxen sodium (NS) in poly(lactide-co-glycolide) (PLGA) (50:50) scaffolds. Efficient chromatographic separation was achieved on a reverse phase column (ODS2 C18 200x4.6mm) with the mobile phase consisted of methanol and acetate buffer (pH 5.1) (55:45 v/v) at a flow rate of 1 mL.min-1 by using fluorescence detector at 254 nm ex/ 352 nm em wavelength. The retention time was about 9.3 min.
more » ... was about 9.3 min. Calibration curve was linear over the concentration range of 0.08-75 ug.mL-1. The intra- and inter-day precision relative standard deviation was 3.71 % or less, and the accuracy was within 2.96 % of the nominal concentration. The developed HPLC method was successfully applied to quantitate NS in PLGA (50:50) scaffolds
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