Evidence for a Second Nitrate Reductase Activity That Is Distinct from the Respiratory Enzyme in Salmonella typhimurium

Ericka L. Barrett, Daniel L. Riggs
1982 Journal of Bacteriology  
Significant nitrate reductase activity was detected in mutants of Salmonella typhimurium which mapped at or near chIC and which were incapable of growth with nitrate as electron acceptor. The same mutants were sensitive to chlorate and performed sufficient nitrate reduction to permit anaerobic growth with nitrate as the sole nitrogen source in media containing glucose. The mutant nitrate-reducing protein did not migrate with the wild-type nitrate reductase in polyacrylamide electrophoretic
more » ... lectrophoretic gels. Studies of the electrophoretic mobility in gels of different polyacrylamide concentration revealed that the wild-type and mutant nitrate to obtain Salmonella mutants which map at chlC, we have never been able to isolate mutants comparable to the E. coli chlC strains on any medium designed for this purpose. On the contrary, in our experience, direct isolation of 563 on May 5, 2020 by guest
doi:10.1128/jb.150.2.563-571.1982 fatcat:kuxzubleaverveio3if43unt6q