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Biosynthesis of Porphyrinogens in Etiolated Euglena gracilis Z. I. Isolation and Purification of an Endogenous Factor Stimulating the Formation of Porphyrinogens

A. A. Juknat, D. Dörnemann, H. Senger
1988 Zeitschrift für Naturforschung C - A Journal of Biosciences  

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A low molecular weight, heat-stable factor has been purified from Euglena gracilis supernatant fraction by employing gel filtration, cation and anion exchange and paper chromatography. This endogenous compound stimulates porphobilinogenase (PBG-ase) (EC 4.3.1.8) activity, an enzyme of the porphyrin biosynthetic pathway. 10-7 ᴍ folic acid and 10-4 ᴍ 6-biopterin produced a significant activation, equivalent to 2-4 units of the purified factor. Elution patterns from the columns and fluorescence
more » ... UV absorption peaks suggest that this compound is a pteridine. This conclusion is further supported by the fact that both, folic acid and 6-biopterin can replace the action of the isolated factor on PBG-ase. The mechanism of stimulation is discussed.
doi:10.1515/znc-1988-5-605 fatcat:etizejyuofe5zjn2z4fc7qiuyu
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A. A. Juknat, D. Dörnemann, H. Senger. "Biosynthesis of Porphyrinogens in Etiolated Euglena gracilis Z. I. Isolation and Purification of an Endogenous Factor Stimulating the Formation of Porphyrinogens." Zeitschrift für Naturforschung C - A Journal of Biosciences 43.5-6 (1988) 351-356