It is known that Kv1.3, KCa3.1, CRAC, TRPM7, and Clswell are five im por tant ion channels in T lym pho cytes. To in ves ti gate the mech a nism un der ly ing the pro tec tive ef fects of lentinan against T lym pho cytes in jury in duced by chronic ra di a tion in mice, we de ter mined the ex pres sion pro files of Kv1.3, KCa3.1, Orai1, STIM1, TRPM7, and Clswell genes. Mice were in jected with dif fer ent con cen tra tions of lentinan be fore ex po sure to 60 Co g-ray. To tal RNA was then ex

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... cted from T lym pho cytes in murine spleen. The Real time-PCR was performed to de tect the ex pres sion lev els of Kv1.3, KCa3.1, Orai1, STIM1, TRPM7, and Clswell genes. The re sults show that, out of the six ion chan nels gene ex pres sion de tected in the pres ent study, only Kv1.3 and Orai1 were al tered by lentinan in mice un der chronic ra di ation stress. Kv1.3 and Orai1 ex pres sion were sig nif i cantly el e vated in T-lym pho cytes iso lated from ir ra di ated mice, and were nor mal ized by lentinan treat ment. Our re sults sug gest that that Kv1.3 and Orai1 chan nels may me di ate the anti-ra di a tion ef fects of lentinan.