The dried rhizome of Curcuma longa was used for isolation of Isoflavone by using the extraction process known as maceration in which the rhizomes were macerated in hydro-alcoholic solvent in proportion (1:1). The extraction was carried out for 15 days. The dark yellow colored extract obtained after extraction was sub fractioned by chloroform and the isoflavone was obtained which was confirmed by Shinoda test for flavonoids. The extract (isoflavone) was subjected to carry out the antibacterial

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... tivity. The activity was carried out for different concentrations and solvent (DMSO) taken as blank using agar diffusion technique by cup plate method. The extracts were added at concentration of 500 μg/ml, 250μg/ml, 125μg/ml, 62.5μg/ml, 31.25μg/ml into the five wells. The plates were kept in incubator for 18-24hr at 37ºC, zones of inhibitions were measured and compared with the standard drugs.