Effect of prochloraz fungicide on biotransformation enzymes and oxidative stress parameters in three-spined stickleback (Gasterosteus aculeatus L.)

Wilfried Sanchez, Benjamin Piccini, Jean-Marc Porcher
2007 Journal of Environmental Science and Health. Part B - Pesticides, Food Contaminants, and Agricultural Wastes  
The aim of this study was to characterise biomarker responses in three-spined sticklebacks exposed to prochloraz (Pcz). For this purpose, adult sticklebacks were exposed for 2 weeks to prochloraz at 0, 10, 50, 100 and 500 µg/L prior to one week of depuration in clean water. At days 7, 14 and 21, several hepatic biomarkers were measured including 7-ethoxyresorufin-Odeethylase (EROD), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), total glutathione (GSH) content
more » ... ne (GSH) content and thiobarbituric acid reactive substances (TBARS). Pcz induced a transient increase of antioxidant enzymes and a depletion of glutathione content during the first 7 days of exposure. This study showed that EROD activity and antioxidants were disrupted in a transient manner. GST was rapidly induced in a dose-dependent manner and this induction was persitent and observed also after depuration. GST appeared as a valuable biomarker to assess the exposure to Pcz.
doi:10.1080/03601230701735151 pmid:18161575 fatcat:th7qkzfjr5asrbaccnhk74inhy