GC-MS Analysis of Fungal Filtrate Crude Extracts Produced by Two Species of Aspergillus

Dhurgham Alhasan, Ahmed Dawood, Hind Alfartossy
2019 Journal of College of Education for Pure Science  
The species of the genus Aspergillus have an ability to produce secondary metabolites, however, not all filamentous fungi can form the metabolites. The current study showed that two species of the genus grew at 27 °C on potato dextrose agar (PDA). Aspergillus terreus appeared brown colonies on the PDA but A. niger grew as black colonies. Potato dextrose broth (PDB) used to be a fermenting medium by which the fungal filtrate of both fungi separated from the mycelia. Residues of the extracted
more » ... f the extracted filtrates gave the blackish brown crude extracts. The extract weights recorded 300 mg/L obtained from A. niger, and 200 mg/L of A. terreus. GC-MS analysis revealed the extract of A. niger consists of three compounds are {[5-methyl-2-phenylindolizine],[thiocarbamicacid,N,Ndimethyl,S-1,3-diphenyl-2-butenylester], and [22-beta.-acetoxy-3.beta.,16.alpha.-dihydroxy13,28 epoxyolean-2]}. A. terreus extract possesses four compounds are {[2.2] paracyclophane, [5-methyl-2-phenylindolizine],[2H -1-benzopyran-6-ol,3,4dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltride)] , and [dI-.alpha. tocopherol]}. 300 μL taken from 10 mg/ml of A. niger crude extract led to hemolysis of red blood cells while 800 μL of the same concentration from A. terreus extract gave the hemolysis. The present study concluded that 5-methyl-2phenylindolizine was detected a similar compound in crude extracts of both A.niger and A.terreus with the same retention time. It may be attributed to the presenting same gene within the genome of both fungi led to producing 5-methyl-2phenylindolizine.
doi:10.32792/utq.jceps.09.01.27 fatcat:rf6nnqvk3bdm5fn4g5eguvnbwi